1. After the efforts and improvements of our great predecessors, the optical microscope has reached a perfect level. In fact, ordinary microscopes can provide us with beautiful microscopic images simply and quickly. However, the event that brought revolutionary innovation to this almost perfect microscope world occurred, which was the invention of the "laser scanning confocal microscope". The characteristic of this new type of microscope is that it adopts an optical system that only extracts image information from the surface where the focus is concentrated. By changing the focus while restoring the obtained information in the image memory, it can obtain a vivid image with complete three-dimensional information intelligence. Through this method, information about surface shape that cannot be confirmed by conventional microscopes can be easily obtained. In addition, for typical optical microscopes, "improving resolution" and "deepening focal depth" are contradictory conditions, especially at high magnification. However, in confocal microscopes, this problem is easily solved.
2. Advantages of confocal optical systems
Sistemul confocal optic este utilizat pentru a ilumina eșantionul cu puncte% 2c în timp ce reflectat lumina este de asemenea primit de a punct senzor. Când eșantionul este plasat la punctul focal% 2c aproape tot din reflectat lumină poate ajunge dispozitivul fotosensibil Când eșantionul deviază de la punctul focal% 2c reflectat lumină nu poate ajunge dispozitivul fotosensibil . That is to say, in a confocal optical system, only images that coincide with the focal point will be output, and the light spot and useless scattered light will be shielded.
Why use laser?
In a confocal optical system, the sample is illuminat and the reflected light is also received by a point sensor. Prin urmare, point light sources become necessary. Laser is a foy point light source. In most cases, the light source of a confocal microscop is a laser light source. In addition, the monochromaticity, directionality, and excelent beam shape of lasers are also important reasons thelor widespread adoption.
4. Timp real observație bazat pe viteză mare scanare devine posibil
Proiectul laser scanare utilizare acustic optic deflector (AO prime) in the orizontal direction and Servo Galvano mirror in the the vertical direction. Due to the absence of mechanical vibrations in the audio optical bias unit, it is possible to perform high-speed scanning and observe in real-time on the monitoring screen. The high-speed nature of this type of camera is a foart important item that directly afects the speed of focusing and position Regăsire.
5. Relație între focal poziție și luminozitate
In a confocal optical system, the brightness of the sample is high when it is correct placed in the focal position, and its brightness lowers sharply before and after it (solid line in Figure 4). The sensitive selectivity of this focal plane is precise the the principle behind the determination of height direction and the expansion of focal depth in confocal microscopy. Comparat cu acest, tipic optic microscoape do not show semnificativ luminozitate modificări înainte și după the focal position (punctat linie in figură 4).
6. Ridicat contrast și rezoluție
